Winuthayanon Lab

Protocols

Lab Notebook Record

Analyze scRNA-seq data

Mouse IVF protocol

Single nuclei isolation from frozen mouse oviducts

Periodic Acid Schiff (PAS) Staining (from IHC World)

Neutrophil and NETs IF staining

MUCIN1 (MUC1) IHC staining

Masson's Trichrome Staining (from IHC World)

EdU staining

MUCIN9 (OVGP1) IHC staining

ESR1 IHC staining

PGR IHC staining

Ki67 IHC staining

Acetylated-a-tubulin IHC staining

Acetylated-a-tubulin IF staining

B-Catenin IHC staining

H&E staining

Vaginal smear staining

PAX8 IHC staining

Collecting pre-implantation embryos

Acrosome reaction staining

How to use cryostat

General info on cell culture

Embedding and sectioning tissues

Counting cell using hemocytometer

picture credit: http://microbiology.ucdavis.edu/privalsky/hemocytometer

Grams Staining (from HIMEDIA Lab)

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