Transcriptomics + Proteomics data
scRNA-seq, Bulk RNA-seq, Proteomic Datasets
https://genes.winuthayanon.com/winuthayanon/ to access all datasets in our lab.
All of our sequencing and proteomics data are available for public. Visit to search for your favorite genes in our datasets!
All of source codes for estrus, estrogen treatment, and human fallopian tube data analysis are deposited on Github.
All raw sequencing data are deposited on NCBI GSE164291, GSE244422, and GSE270654.
Proteomic analysis in EVs from luminal fluid - mouse oviduct during early pregnancy
Extracellular vesicles (EVs) were isolated from the oviductal luminal fluid at estrus, superovulation, 0.5 dpc, 1.5 dpc, and 2.5 dpc using EV trap method in the C57BL6 mouse model. Proteins were identified using LC/MS-MS analysis. The absence of a protein (indicated by a "0" value) does not necessarily mean it was not present in the sample. These proteins may be present at levels too low to be detected by our current methodology. Search your protein here. Unpublished.
Proteomic analysis from luminal fluid - mouse oviduct during early pregnancy
Proteins were isolated from the oviductal luminal fluid at estrus, 0.5 dpc, 1.5 dpc, and 2.5 dpc with or without superovulation with 5 U of Pregnant's mare serum gonadotrophin and human chorionic gonadotropin in the C57BL6 mouse model. Proteins were identified using LC/MS-MS analysis. Search your protein here. The dataset is currently linked to this publication in bioRxiv.
Bulk RNA-seq analysis of mouse oviduct during early pregnancy
Oviduct samples were collected from C57BL6 female mice at estrus, 0.5, 1.5, 2.5, and 3.5 days post coitus (dpc). Some females were mated with vasectomized male mice to induce pseudopregnancy. Then the oviducts were collected at 0.5, 1.5, 2.5, and 3.5 days of pseudopregnancy (dpp). Oviducts were dissected into Infundibulum + Ampulla (IA) or Isthmus + Uterotubal Junction (IU) regions. RNA sequencing was performed using Illumina Novaseq. Data were analyzed using Galaxy with trimmomatic, HISAT2 alignment, and featureCounts. n = 3 biological replicates. Search your gene here. The dataset is currently linked to this publication in bioRxiv. GSE270654
Mouse oviduct treated with vehicle or E2 for 2 and 24 h after ovariectomy
Cells from mouse oviducts isolated from ovariectomized (OVX) mice treated with vehicle or 17beta-estradiol (E2) for 2 or 24 hours. To search for the gene(s) expressed in this OVX Vehicle- or E2-treated dataset or to obtain raw data and code for data analysis, please go here. This dataset is currently linked to this publication in Endocrinology.
Mouse oviduct treated with vehicle or 17beta-estradiol for 2 h after ovariectomy
Cells from mouse oviducts isolated from ovariectomized (OVX) mice treated with vehicle or 17beta-estradiol (E2) for 2 hours. To search for the gene(s) expressed in this OVX Vehicle- or E2-treated dataset or to obtain raw data and code for data analysis, please go here. This dataset is currently linked to this publication in Endocrinology.
Mouse oviduct treated with vehicle or 17beta-estradiol for 24h after ovariectomy
Cells from mouse oviducts isolated from ovariectomized (OVX) mice treated with vehicle or 17beta-estradiol (E2) for 24 hours. To search for the gene(s) expressed in this OVX Vehicle- or E2-treated dataset or to obtain raw data and code for data analysis, please go here. This dataset is currently linked to this publication in FASEB J.
